Title : Making the molecular mechanistic movie for CRISPR-Cas12a genetic scissor using single-molecule FRET
Date / Time : 13 Sep, 2022 / 5:00 P.M
Speaker : Sanghwa Lee(Catholic Univ.)
Abstract : My research group aims to make a molecular mechanistic movie for various molecular machines in biological system. To achieve this goal, we have used cutting-edge single-molecule fluorescence techniques, allowing us to observe real-time dynamics of biomolecules. In this talk, I will introduce our recent studies about the action mechanism of CRISPR-Cas12a genetic scissor using single-molecule FRET technique. In these studies, we directly observed the entire process for the DNA cleavage reaction by CRISPR-Cas12a. Our results provide a thorough understanding of the mechanisms that govern the generation of complete double-stranded DNA breaks by a single catalytic site of Cas12a using single-molecule FRET assays to improve our ability to develop a rational design for more potently engineered Cas12a. This would extend the range of applications of Cas12a genetic scissor in the future.